Staphylococcus aureus (SA) food poisoning results from consumption of preformed S. aureus may be important as it is relevant to food hygiene.
Moreover, the high prevalence of toxin-producing S. aureus originating from goats and sheep and bovine milk were found.
Remarkable differences in phenotypic traits between S. aureus strains originating from bulk-tank milk samples of goats and sheep. In summary, within these two groups, high genotypic uniformity within the different sized coagulase gene amplicons was demonstrated.This is the first study providing comprehensive characterisation data of S. Coagulase gene restriction profile analysis of the 145 isolates harbouring SEA or SEC genes revealed six different patterns using AluI and five different patterns using HaeIII. Furthermore, 126 isolates were positive for the gene encoding the toxic shock syndrome toxin 1. In 191 isolates (n=293) staphylococcal enterotoxin (SE) genes were detected: 123 isolates tested positive for SEC gene, 31 for SEG gene, 28 for SEA gene, 26 for SEJ gene, 24 for SEI gene, 4 for SEB gene and 4 for SED gene. Five differently sized PCR products of 500, 580, 660, 740 and 820 bp were distinguished. For 285 isolates, PCR amplification of the 3′ end of the coagulase gene showed a single amplicon. aureus isolates, 193 (65.9%) were egg yolk-negative and 15 (5.1%) were negative for clumping factor and/or protein A determined by a latex agglutinating test system. Two hundred and ninety-three isolates of Staphylococcus aureus obtained from 127 bulk-tank milk samples of goats and sheep from Switzerland were characterised by pheno- and genotypic traits.Of the 293 S.
0 kommentar(er)
